SYBR Green qPCR primers for detecting human FBXW7 (F-box and WD repeat domain containing 7, also called hAgo) gene expression by real-time PCR.
Human FBXW7 qPCR Primer Set – F-box and WD repeat domain containing 7
Also known as: AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
About this gene: FBXW7 belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs, which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.
Gene details: NCBI Gene ID: 55294; HGNC ID: HGNC:16712; UniProt: Q969H0; RefSeq (MANE Select): NM_001349798.2; Chromosome location: 4q31.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.