SYBR Green qPCR primers for detecting human FARSA (phenylalanyl-tRNA synthetase subunit alpha, also called CML33) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human FARSA qPCR Primer Set – phenylalanyl-tRNA synthetase subunit alpha
Also known as: CML33, FARSL, FARSLA, FRSA, PheHA, RILDBC2
About this gene: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARSA encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases. FARSA product is reported to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression.
Gene details: NCBI Gene ID: 2193; HGNC ID: HGNC:3592; UniProt: Q9Y285; RefSeq (MANE Select): NM_004461.3; Chromosome location: 19p13.13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.