SYBR Green qPCR primers for detecting human FAP (fibroblast activation protein alpha, also called SIMP) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human FAP qPCR Primer Set – fibroblast activation protein alpha
Also known as: DPPIV, FAPA, FAPalpha, SIMP
About this gene: The FAP protein is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene product is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. The FAP protein is believed to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.
Gene details: NCBI Gene ID: 2191; HGNC ID: HGNC:3590; UniProt: Q12884; RefSeq (MANE Select): NM_004460.5; Chromosome location: 2q24.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.