SYBR Green qPCR primers for detecting human FANCF (FA complementation group F) gene expression by real-time PCR.
Human FANCF qPCR Primer Set – FA complementation group F
Also known as: FAF
About this gene: The Fanconi anemia complementation group currently includes FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ, FANCL. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. FANCF encodes a protein for complementation group F.
Gene details: NCBI Gene ID: 2188; HGNC ID: HGNC:3587; UniProt: Q9NPI8; RefSeq (MANE Select): NM_022725.4; Chromosome location: 11p14.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.