SYBR Green qPCR primers for detecting human F5 (coagulation factor V) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human F5 qPCR Primer Set – coagulation factor V
Also known as: FVL, PCCF, RPRGL1, THPH2, fV
About this gene: F5 encodes a essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions.
Gene details: NCBI Gene ID: 2153; HGNC ID: HGNC:3542; UniProt: P12259; RefSeq (MANE Select): NM_000130.5; Chromosome location: 1q24.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.