SYBR Green qPCR primers for detecting human EPSTI1 (epithelial stromal interaction 1) gene expression by real-time PCR.
Human EPSTI1 qPCR Primer Set – epithelial stromal interaction 1
Also known as: BRESI1
About this gene: The EPSTI1 protein is reported to promote tumor invasion and metastasis in some invasive cancer cells when overexpressed. Expression of EPSTI1 is reported to be upregulated by direct binding of the Kruppel like factor 8 protein to promoter sequences. The translated protein interacts with the amino terminal region of the valosin containing protein gene product, resulting in the nuclear translocation of the nuclear factor kappa B subunit 1 gene product, and activation of target genes.
Gene details: NCBI Gene ID: 94240; HGNC ID: HGNC:16465; UniProt: Q96J88; RefSeq (MANE Select): NM_033255.5; Chromosome location: 13q14.11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.