SYBR Green qPCR primers for detecting human EHHADH (enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase, also called PBFE) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human EHHADH qPCR Primer Set – enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase
Also known as: ECHD, FRTS3, L-PBE, LBFP, MFE1, PBFE
About this gene: The EHHADH protein is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the EHHADH protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in EHHADH are a cause of peroxisomal disorders such as Zellweger syndrome.
Gene details: NCBI Gene ID: 1962; HGNC ID: HGNC:3247; UniProt: Q08426; RefSeq (MANE Select): NM_001966.4; Chromosome location: 3q27.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.