SYBR Green qPCR primers for detecting human EDEM1 (ER degradation enhancing alpha-mannosidase like protein 1) gene expression by real-time PCR.
Human EDEM1 qPCR Primer Set – ER degradation enhancing alpha-mannosidase like protein 1
Also known as: EDEM
About this gene: EDEM1 enables mannosyl-oligosaccharide 1,2-alpha-mannosidase activity and misfolded protein binding activity. It is involved in multiple processes, including endoplasmic reticulum mannose trimming; positive regulation of retrograde protein transport, ER to cytosol; and protein catabolic process.
Gene details: NCBI Gene ID: 9695; HGNC ID: HGNC:18967; UniProt: Q92611; RefSeq (MANE Select): NM_014674.3; Chromosome location: 3p26.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.