SYBR Green qPCR primers for detecting human EBAG9 (estrogen receptor binding site associated antigen 9) gene expression by real-time PCR.
Human EBAG9 qPCR Primer Set – estrogen receptor binding site associated antigen 9
Also known as: EB9, PDAF
About this gene: EBAG9 was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of EBAG9. The EBAG9 protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers.
Gene details: NCBI Gene ID: 9166; HGNC ID: HGNC:3123; UniProt: O00559; RefSeq (MANE Select): NM_004215.5; Chromosome location: 8q23.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.