SYBR Green qPCR primers for detecting human DPH1 (diphthamide biosynthesis 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human DPH1 qPCR Primer Set – diphthamide biosynthesis 1
Also known as: DEDSSH, DPH2L, DPH2L1, OVCA1
About this gene: The DPH1 protein is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2. Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in DPH1 have been linked to both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
Gene details: NCBI Gene ID: 1801; HGNC ID: HGNC:3003; UniProt: Q9BZG8; RefSeq (MANE Select): NM_001383.6; Chromosome location: 17p13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.