SYBR Green qPCR primers for detecting human AKAP7 (A-kinase anchoring protein 7) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human AKAP7 qPCR Primer Set – A-kinase anchoring protein 7
Also known as: AKAP15, AKAP18
About this gene: AKAP7 belongs to the A-kinase anchoring protein family, a group of functionally related proteins that bind to a regulatory subunit of cAMP-dependent protein kinase A and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations.
Gene details: NCBI Gene ID: 9465; HGNC ID: HGNC:377; UniProt: O43687; RefSeq (MANE Select): NM_016377.4; Chromosome location: 6q23.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
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