SYBR Green qPCR primers for detecting human DMD (dystrophin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human DMD qPCR Primer Set – dystrophin
Also known as: BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85
About this gene: DMD spans a genomic range of greater than 2 Mb and encodes a large protein containing an N-terminal actin-binding domain and multiple spectrin repeats. The DMD protein forms a component of the dystrophin-glycoprotein complex, which bridges the inner cytoskeleton and the extracellular matrix. Deletions, duplications, and point mutations at DMD locus may cause Duchenne muscular dystrophy, Becker muscular dystrophy, or cardiomyopathy.
Gene details: NCBI Gene ID: 1756; HGNC ID: HGNC:2928; UniProt: P11532; RefSeq (MANE Select): NM_004006.3; Chromosome location: Xp21.2-p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.