SYBR Green qPCR primers for detecting human DLD (dihydrolipoamide dehydrogenase) gene expression by real-time PCR.
Human DLD qPCR Primer Set – dihydrolipoamide dehydrogenase
Also known as: DLDD, DLDH, E3, GCSL, LAD, OGDC-E3, PHE3
About this gene: DLD belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. The DLD protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the DLD protein acts as a dehydrogenase and is found in multiple multi-enzyme complexes that regulate energy metabolism.
Gene details: NCBI Gene ID: 1738; HGNC ID: HGNC:2898; UniProt: P09622; RefSeq (MANE Select): NM_000108.5; Chromosome location: 7q31.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.