SYBR Green qPCR primers for detecting human DKK1 (dickkopf Wnt signaling pathway inhibitor 1, also called SK) gene expression by real-time PCR.
Human DKK1 qPCR Primer Set – dickkopf Wnt signaling pathway inhibitor 1
Also known as: DKK-1, SK
About this gene: DKK1 belongs to the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The DKK1 protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling.
Gene details: NCBI Gene ID: 22943; HGNC ID: HGNC:2891; UniProt: O94907; RefSeq (MANE Select): NM_012242.4; Chromosome location: 10q21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.