SYBR Green qPCR primers for detecting human DCTN3 (dynactin subunit 3, also called p22) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human DCTN3 qPCR Primer Set – dynactin subunit 3
Also known as: DCTN-22, DCTN22
About this gene: DCTN3 encodes a smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. The DCTN3 gene product is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. The DCTN3 gene product is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit of dynactin.
Gene details: NCBI Gene ID: 11258; HGNC ID: HGNC:2713; UniProt: O75935; RefSeq (MANE Select): NM_007234.5; Chromosome location: 9p13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.