SYBR Green qPCR primers for detecting human DCTD (dCMP deaminase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human DCTD qPCR Primer Set – dCMP deaminase
About this gene: The DCTD protein catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The DCTD protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. The DCTD protein uses zinc as a cofactor for its activity.
Gene details: NCBI Gene ID: 1635; HGNC ID: HGNC:2710; UniProt: P32321; RefSeq (MANE Select): NM_001921.3; Chromosome location: 4q35.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.