SYBR Green qPCR primers for detecting human CTH (cystathionine gamma-lyase, also called CGL) gene expression by real-time PCR.
Human CTH qPCR Primer Set – cystathionine gamma-lyase
Also known as: CGL, CSE
About this gene: CTH encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in CTH cause cystathioninuria.
Gene details: NCBI Gene ID: 1491; HGNC ID: HGNC:2501; UniProt: P32929; RefSeq (MANE Select): NM_001902.6; Chromosome location: 1p31.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.