SYBR Green qPCR primers for detecting human CSPG4 (chondroitin sulfate proteoglycan 4) gene expression by real-time PCR.
Human CSPG4 qPCR Primer Set – chondroitin sulfate proteoglycan 4
Also known as: CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
About this gene: A human melanoma-associated chondroitin sulfate proteoglycan is involved in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Gene details: NCBI Gene ID: 1464; HGNC ID: HGNC:2466; UniProt: Q6UVK1; RefSeq (MANE Select): NM_001897.5; Chromosome location: 15q24.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.