SYBR Green qPCR primers for detecting human CRB1 (crumbs cell polarity complex component 1) gene expression by real-time PCR.
Human CRB1 qPCR Primer Set – crumbs cell polarity complex component 1
Also known as: CRB1-A, CRB1-B, CRB1-C, LCA8, RP12
About this gene: CRB1 encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in CRB1 are linked to a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis.
Gene details: NCBI Gene ID: 23418; HGNC ID: HGNC:2343; UniProt: P82279; RefSeq (MANE Select): NM_201253.3; Chromosome location: 1q31.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.