SYBR Green qPCR primers for detecting human AFP (alpha fetoprotein) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human AFP qPCR Primer Set – alpha fetoprotein
Also known as: AFPD, FETA, HPAFP
About this gene: AFP encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often linked to hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology.
Gene details: NCBI Gene ID: 174; HGNC ID: HGNC:317; UniProt: P02771; RefSeq (MANE Select): NM_001134.3; Chromosome location: 4q13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.