SYBR Green qPCR primers for detecting human KNL1 (kinetochore scaffold 1, also called CT29) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human KNL1 qPCR Primer Set – kinetochore scaffold 1
Also known as: AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105
About this gene: The KNL1 protein is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The KNL1 protein acts as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, KNL1 is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues.
Gene details: NCBI Gene ID: 57082; HGNC ID: HGNC:24054; UniProt: Q8NG31; RefSeq (MANE Select): NM_144508.5; Chromosome location: 15q15.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.