SYBR Green qPCR primers for detecting human CLSPN (claspin, also called hClaspin) gene expression by real-time PCR.
Human CLSPN qPCR Primer Set – claspin
About this gene: The product of CLSPN is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase.
Gene details: NCBI Gene ID: 63967; HGNC ID: HGNC:19715; UniProt: Q9HAW4; RefSeq (MANE Select): NM_022111.4; Chromosome location: 1p34.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.