SYBR Green qPCR primers for detecting human CHKB (choline kinase beta, also called CK) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CHKB qPCR Primer Set – choline kinase beta
Also known as: CHETK, CHKL, CK, CKB, CKEKB, EK, EKB, MDCMC
About this gene: Choline kinase and ethanolamine kinase catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein.
Gene details: NCBI Gene ID: 1120; HGNC ID: HGNC:1938; UniProt: Q9Y259; RefSeq (MANE Select): NM_005198.5; Chromosome location: 22q13.33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.