SYBR Green qPCR primers for detecting human CFL1 (cofilin 1, also called p18) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CFL1 qPCR Primer Set – cofilin 1
Also known as: CFL, HEL-S-15, cofilin
About this gene: The CFL1 protein can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.
Gene details: NCBI Gene ID: 1072; HGNC ID: HGNC:1874; UniProt: P23528; RefSeq (MANE Select): NM_005507.3; Chromosome location: 11q13.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.