SYBR Green qPCR primers for detecting human CDKN1B (cyclin dependent kinase inhibitor 1B, also called p27Kip1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human CDKN1B qPCR Primer Set – cyclin dependent kinase inhibitor 1B
Also known as: CDKN4, KIP1, MEN1B, MEN4, P27KIP1
About this gene: CDKN1B encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The CDKN1B protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Gene details: NCBI Gene ID: 1027; HGNC ID: HGNC:1785; UniProt: P46527; RefSeq (MANE Select): NM_004064.5; Chromosome location: 12p13.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.