SYBR Green qPCR primers for detecting human NUF2 (NUF2 component of NDC80 kinetochore complex, also called hNuf2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NUF2 qPCR Primer Set – NUF2 component of NDC80 kinetochore complex
Also known as: CDCA1, CT106, NUF2R
About this gene: NUF2 encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex linked to the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The NUF2 protein is found to be linked to centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2.
Gene details: NCBI Gene ID: 83540; HGNC ID: HGNC:14621; UniProt: Q9BZD4; RefSeq (MANE Select): NM_145697.3; Chromosome location: 1q23.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.