SYBR Green qPCR primers for detecting human CBFA2T2 (CBFA2/RUNX1 partner transcriptional co-repressor 2, also called p85) gene expression by real-time PCR.
Human CBFA2T2 qPCR Primer Set – CBFA2/RUNX1 partner transcriptional co-repressor 2
Also known as: EHT, MTGR1, ZMYND3, p85
About this gene: In acute myeloid leukemia, especially in the M2 subtype, the t translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of the CBFA2T1 gene. The chimeric protein is believed to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.
Gene details: NCBI Gene ID: 9139; HGNC ID: HGNC:1536; UniProt: O43439; RefSeq (MANE Select): NM_001032999.3; Chromosome location: 20q11.21-q11.22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.