SYBR Green qPCR primers for detecting human ADA (adenosine deaminase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ADA qPCR Primer Set – adenosine deaminase
Also known as: ADA1
About this gene: ADA encodes a enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for ADA and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity.
Gene details: NCBI Gene ID: 100; HGNC ID: HGNC:186; UniProt: P00813; RefSeq (MANE Select): NM_000022.4; Chromosome location: 20q13.12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.