SYBR Green qPCR primers for detecting human CEP19 (centrosomal protein 19) gene expression by real-time PCR.
Human CEP19 qPCR Primer Set – centrosomal protein 19
Also known as: C3orf34, MOSPGF
About this gene: The CEP19 protein localizes to centrosomes and primary cilia and co-localizes with a marker for the mother centriole. CEP19 resides in a region of human chromosome 3 that is linked to morbid obesity. A homozygous knockout of the orthologous gene in mouse resulted in mice with morbid obesity, hyperphagy, glucose intolerance, and insulin resistance.
Gene details: NCBI Gene ID: 84984; HGNC ID: HGNC:28209; UniProt: Q96LK0; RefSeq (MANE Select): NM_032898.5; Chromosome location: 3q29
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.