SYBR Green qPCR primers for detecting human YTHDF1 (YTH N6-methyladenosine RNA binding protein F1, also called DF1) gene expression by real-time PCR.
Human YTHDF1 qPCR Primer Set – YTH N6-methyladenosine RNA binding protein F1
Also known as: C20orf21, DF1
About this gene: YTHDF1 enables N6-methyladenosine-containing RNA reader activity and ribosome binding activity. It is involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Implicated in colorectal cancer.
Gene details: NCBI Gene ID: 54915; HGNC ID: HGNC:15867; UniProt: Q9BYJ9; RefSeq (MANE Select): NM_017798.4; Chromosome location: 20q13.33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.