Human PPIA qPCR primer set for peptidylprolyl isomerase A (cyclophilin A)

SYBR Green qPCR primers for detecting human PPIA (peptidylprolyl isomerase A, also called cyclophilin A) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human PPIA qPCR Primer Set – peptidylprolyl isomerase A

Also known as: CYPA, CYPH, HEL-S-69p, cyclophilin A

About this gene: PPIA belongs to the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The PPIA protein is a cyclosporin-binding protein and may be involved in cyclosporin A-mediated immunosuppression, and it is also reported to interact with several HIV proteins.

Gene details: NCBI Gene ID: 5478; HGNC ID: HGNC:9253; UniProt: P62937; RefSeq (MANE Select): NM_021130.5; Chromosome location: 7p13

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets, or profile PPIA alongside related genes with our Housekeeping Gene Primer Sets.

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