Rat Ubiquitin Metabolism Primer Library

Contains 88 primer sets directed against ubiquitin metabolism genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix



GENE LIST:

Anapc2 anaphase promoting complex subunit 2

Anapc11 anaphase promoting complex subunit 11

Arih1 ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1

Atg7 ATG7 autophagy related 7 homolog

Bard1 BRCA1 associated RING domain 1

Brca1 Breast cancer 1, early onset

Brca2 Breast cancer 2, early onset

Brcc3 BRCA1/BRCA2-containing complex, subunit 3

Btrc beta-transducin repeat containing

Cbl Cas-Br-M (murine) ecotropic retroviral transforming sequence

Cdc34 cell division cycle 34 homolog

Cul1 cullin 1

Cul2 cullin 2

Cul3 cullin 3

Cul4b cullin 4B

Cul5 cullin 5

Ddb1 damage-specific DNA binding protein 1, 127kDa

Fbxo11 F-box protein 11

Fbxo32 F-box protein 32

Fbxo4 F-box protein 4

Fbxo6 F-box protein 6

Fbxw9 F-box and WD repeat domain containing 9

Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2

Itch itchy E3 ubiquitin protein ligase homolog (mouse)

March5 membrane-associated ring finger (C3HC4) 5

Mdm2 Mdm2 p53 binding protein homolog

Mib1 mindbomb homolog 1 (Drosophila)

Mocs3 molybdenum cofactor synthesis 3

Mul1 mitochondrial E3 ubiquitin protein ligase 1

Nae1 NEDD8 activating enzyme E1 subunit 1

Nedd8 neural precursor cell expressed, developmentally down-regulated 8

Park2 Parkinson disease (autosomal recessive, juvenile) 2, parkin

Rfwd2 ring finger and WD repeat domain 2

Rlim ring finger protein, LIM domain interacting

Rnf144b ring finger protein 144B

Rnf167 ring finger protein 167

Rnf180 ring finger protein 180

Rnf2 ring finger protein 2

Rnf20 ring finger protein 20

Rnf34 ring finger protein 34

Rnf40 ring finger protein 40

Rnf6 ring finger protein (C3H2C3 type) 6

Rnf8 ring finger protein 8

Sae1 SUMO1 activating enzyme subunit 1

Skp1 S-phase kinase-associated protein 1

Skp2 S-phase kinase-associated protein 2 (p45)

Smurf1 SMAD specific E3 ubiquitin protein ligase 1

Smurf2 SMAD specific E3 ubiquitin protein ligase 2

Stub1 STIP1 homology and U-Box containing protein 1

Syvn1 synovial apoptosis inhibitor 1, synoviolin

Tmem189 transmembrane protein 189

Tp53 tumor protein p53

Uba1 ubiquitin-like modifier activating enzyme 1

Uba2 ubiquitin-like modifier activating enzyme 2

Uba3 ubiquitin-like modifier activating enzyme 3

Uba5 ubiquitin-like modifier activating enzyme 5

Uba6 ubiquitin-like modifier activating enzyme 6

Ube2a ubiquitin-conjugating enzyme E2A

Ube2b ubiquitin-conjugating enzyme E2B

Ube2c ubiquitin-conjugating enzyme E2C

Ube2d1 ubiquitin-conjugating enzyme E2D 1

Ube2d3 ubiquitin-conjugating enzyme E2D 3

Ube2e2 ubiquitin-conjugating enzyme E2E 2

Ube2e3 ubiquitin-conjugating enzyme E2E 3

Ube2g1 ubiquitin-conjugating enzyme E2G 1

Ube2g2 ubiquitin-conjugating enzyme E2G 2

Ube2i ubiquitin-conjugating enzyme E2I

Ube2j1 ubiquitin-conjugating enzyme E2, J1

Ube2j2 ubiquitin-conjugating enzyme E2, J2

Ube2k ubiquitin-conjugating enzyme E2K

Ube2l3 ubiquitin-conjugating enzyme E2L 3

Ube2l6 ubiquitin-conjugating enzyme E2L 6

Ube2m ubiquitin-conjugating enzyme E2M

Ube2n ubiquitin-conjugating enzyme E2N

Ube2q1 ubiquitin-conjugating enzyme E2Q family member 1

Ube2q2 ubiquitin-conjugating enzyme E2Q family member 2

Ube2q2l ubiquitin-conjugating enzyme E2Q family member 2-like

Ube2s ubiquitin-conjugating enzyme E2S

Ube2t ubiquitin-conjugating enzyme E2T (putative)

Ube2v1 ubiquitin-conjugating enzyme E2 variant 1

Ube2v2 ubiquitin-conjugating enzyme E2 variant 2

Ube2z ubiquitin-conjugating enzyme E2Z

Ube4a ubiquitination factor E4A

Uchl1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

Uchl3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

Uchl5 ubiquitin carboxyl-terminal hydrolase L5

Vhl von Hippel-Lindau tumor suppressor

Wwp1 WW domain containing E3 ubiquitin protein ligase 1

Actb Actin, beta

B2m Beta-2-microglobulin

Gapd Glyceraldehyde-3-phosphate dehydrogenase

Gusb Glucuronidase, beta

Hprt1 Hypoxanthine phosphoribosyltransferase 1

Pgk Phosphoglycerate kinase 1

Ppia Peptidylprolyl isomerase A

Rpl13a Ribosomal protein L13a