Rat Gapdh qPCR primer set for glyceraldehyde-3-phosphate dehydrogenase

SYBR Green qPCR primers for detecting rat Gapdh (glyceraldehyde-3-phosphate dehydrogenase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Rat Gapdh qPCR Primer Set – glyceraldehyde-3-phosphate dehydrogenase

Also known as: BARS-38, Gapd

About this gene: NCBI Gene states that rat Gapdh encodes a member of the glyceraldehyde-3-phosphate dehydrogenase family. A similar protein in human and mouse has been identified as a moonlighting protein with mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned additional functions, including nitrosylation of nuclear proteins. Many similar pseudogenes are found throughout the rat genome. Rat Gapdh is commonly used as a reference (housekeeping) gene to normalize qPCR data from rat cells and tissues, and it is also a target in studies of glycolysis.

Gene details: NCBI Gene ID: 24383; UniProt: P04797; Chromosome location: 4q42

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Rat PCR Primer Sets, or profile Gapdh alongside related genes with our Housekeeping Gene Primer Sets.

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