Rat Actb qPCR primer set for actin, beta (beta-actin)

SYBR Green qPCR primers for detecting rat Actb (actin, beta, also called beta-actin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Rat Actb qPCR Primer Set – actin, beta

Also known as: Actx

About this gene: NCBI Gene, drawing on Alliance of Genome Resources annotation, reports that rat Actb enables protein kinase binding activity and is involved in several processes, including cellular response to electrical stimulus, regulation of norepinephrine uptake, and response to immobilization stress. The protein is located in the actin cytoskeleton, axon, and membrane raft, and is part of a protein-containing complex. It has been used to study metabolic dysfunction-associated steatotic liver disease and is a biomarker of temporal lobe epilepsy. The human ortholog is implicated in Baraitser-Winter syndrome 1 and thrombocytopenia. As in other vertebrates, beta-actin is a core cytoskeletal protein involved in cell structure and motility. Rat Actb is widely used as a reference (housekeeping) gene for normalizing qPCR data from rat cells and tissues.

Gene details: NCBI Gene ID: 81822; UniProt: P60711; Chromosome location: 12p11

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Rat PCR Primer Sets, or profile Actb alongside related genes with our Housekeeping Gene Primer Sets.

Need Master Mix?