Nuclear receptors are ligand activated transcription factors that respond to steroid hormones, thyroid hormone, retinoids, vitamin D, fatty acids and other lipophilic molecules. The family includes receptors such as ESR1, AR, GR (NR3C1), PR, THR, RAR, RXR, VDR, PPARs, LXR and FXR. When bound to ligand, nuclear receptors bind hormone response elements in DNA and recruit coactivators (such as NCOA1-3 and CBP/p300) or corepressors (NCOR1, NCOR2) to switch genes on or off. Nuclear receptors regulate development, metabolism and reproduction and are major drug targets.
A ready-to-use Human Nuclear Receptors & Coregulators PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against human nuclear receptor and coregulator genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix Gene List: AHR aryl hydrocarbon receptor AR androgen receptor ARNT aryl hydrocarbon receptor nuclear translocator BRD8 bromodomain containing 8 COPS2 COP9 signalosome subunit 2 CREBBP CREB binding protein DDX5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 ESR1 estrogen receptor 1 ESR2 estrogen receptor 2 (ER beta) ESRRA estrogen-related receptor alpha ESRRB estrogen-related receptor beta ESRRG estrogen-related receptor gamma HDAC1 histone deacetylase 1 HDAC2 histone deacetylase 2 HDAC3 histone deacetylase 3 HDAC4 histone deacetylase 4 HDAC5 histone deacetylase 5 HDAC6 histone deacetylase 6 HDAC7 histone deacetylase 7 HMGA1 high mobility group AT-hook 1 HNF4A hepatocyte nuclear factor 4, alpha KAT5 K(lysine) acetyltransferase 5 ITGB3BP integrin beta 3 binding protein (beta3-endonexin) MED1 mediator complex subunit 1 MED12 mediator complex subunit 12 MED13 mediator complex subunit 13 MED14 mediator complex subunit 14 MED16 mediator complex subunit 16 MED17 mediator complex subunit 17 MED24 mediator complex subunit 24 MED4 mediator complex subunit 4 MTA1 metastasis associated 1 NCOA1 nuclear receptor coactivator 1 NCOA2 nuclear receptor coactivator 2 NCOA3 nuclear receptor coactivator 3 NCOA4 nuclear receptor coactivator 4 NCOA6 nuclear receptor coactivator 6 NCOR1 nuclear receptor corepressor 1 NCOR2 nuclear receptor corepressor 2 NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B- cells 2 (p49/p100) NONO non-POU domain containing, octamer-binding NOTCH2 notch 2 NR0B1 nuclear receptor subfamily 0, group B, member 1 NR0B2 nuclear receptor subfamily 0, group B, member 2 NR1D1 nuclear receptor subfamily 1, group D, member 1 NR1D2 nuclear receptor subfamily 1, group D, member 2 NR1H2 nuclear receptor subfamily 1, group H, member 2 NR1H3 nuclear receptor subfamily 1, group H, member 3 NR1H4 nuclear receptor subfamily 1, group H, member 4 NR1I2 nuclear receptor subfamily 1, group I, member 2 NR1I3 nuclear receptor subfamily 1, group I, member 3 NR2C1 nuclear receptor subfamily 2, group C, member 1 NR2C2 nuclear receptor subfamily 2, group C, member 2 NR2E1 nuclear receptor subfamily 2, group E, member 1 NR2E3 nuclear receptor subfamily 2, group E, member 3 NR2F1 nuclear receptor subfamily 2, group F, member 1 NR2F2 nuclear receptor subfamily 2, group F, member 2 NR2F6 nuclear receptor subfamily 2, group F, member 6 NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) NR3C2 nuclear receptor subfamily 3, group C, member 2 NR4A1 nuclear receptor subfamily 4, group A, member 1 NR4A2 nuclear receptor subfamily 4, group A, member 2 NR4A3 nuclear receptor subfamily 4, group A, member 3 NR5A1 nuclear receptor subfamily 5, group A, member 1 NR5A2 nuclear receptor subfamily 5, group A, member 2 NR6A1 nuclear receptor subfamily 6, group A, member 1 NRIP1 nuclear receptor interacting protein 1 KAT2B K(lysine) acetyltransferase 2B PPARA peroxisome proliferator-activated receptor alpha PPARD peroxisome proliferator-activated receptor delta PPARG peroxisome proliferator-activated receptor gamma PPARGC1A peroxisome proliferator-activated receptor gamma, coactivator 1 alpha PPARGC1B peroxisome proliferator-activated receptor gamma, coactivator 1 beta PSMC3 proteasome (prosome, macropain) 26S subunit, ATPase, 3 PSMC5 proteasome (prosome, macropain) 26S subunit, ATPase, 5 RARA retinoic acid receptor, alpha RARB retinoic acid receptor, beta RARG retinoic acid receptor, gamma RBPJ recombination signal binding protein for immunoglobulin kappa J region RORA RAR-related orphan receptor A RORB RAR-related orphan receptor B RORC RAR-related orphan receptor C RXRA retinoid X receptor, alpha RXRB retinoid X receptor, beta RXRG retinoid X receptor, gamma THRA thyroid hormone receptor, alpha THRB thyroid hormone receptor, beta TRIP4 thyroid Hormone Receptor Interactor 4 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 Pgk1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $595.00 |
A ready-to-use Mouse Nuclear Receptors & Coregulators PCR primer library that may be used to set up to 100 PCR arrays. Contains 88 primer sets directed against mouse nuclear receptor and coregulator genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (40 ul - 10 uM) Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction) Just add cDNA template and SYBR green master mix Gene List: AHR aryl hydrocarbon receptor AR androgen receptor ARNT aryl hydrocarbon receptor nuclear translocator BRD8 bromodomain containing 8 COPS2 COP9 signalosome subunit 2 CREBBP CREB binding protein DDX5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 ESR1 estrogen receptor 1 ESR2 estrogen receptor 2 (ER beta) ESRRA estrogen-related receptor alpha ESRRB estrogen-related receptor beta ESRRG estrogen-related receptor gamma HDAC1 histone deacetylase 1 HDAC2 histone deacetylase 2 HDAC3 histone deacetylase 3 HDAC4 histone deacetylase 4 HDAC5 histone deacetylase 5 HDAC6 histone deacetylase 6 HDAC7 histone deacetylase 7 HMGA1 high mobility group AT-hook 1 HNF4A hepatocyte nuclear factor 4, alpha KAT5 K(lysine) acetyltransferase 5 ITGB3BP integrin beta 3 binding protein (beta3-endonexin) MED1 mediator complex subunit 1 MED12 mediator complex subunit 12 MED13 mediator complex subunit 13 MED14 mediator complex subunit 14 MED16 mediator complex subunit 16 MED17 mediator complex subunit 17 MED24 mediator complex subunit 24 MED4 mediator complex subunit 4 MTA1 metastasis associated 1 NCOA1 nuclear receptor coactivator 1 NCOA2 nuclear receptor coactivator 2 NCOA3 nuclear receptor coactivator 3 NCOA4 nuclear receptor coactivator 4 NCOA6 nuclear receptor coactivator 6 NCOR1 nuclear receptor corepressor 1 NCOR2 nuclear receptor corepressor 2 NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B- cells 2 (p49/p100) NONO non-POU domain containing, octamer-binding NOTCH2 notch 2 NR0B1 nuclear receptor subfamily 0, group B, member 1 NR0B2 nuclear receptor subfamily 0, group B, member 2 NR1D1 nuclear receptor subfamily 1, group D, member 1 NR1D2 nuclear receptor subfamily 1, group D, member 2 NR1H2 nuclear receptor subfamily 1, group H, member 2 NR1H3 nuclear receptor subfamily 1, group H, member 3 NR1H4 nuclear receptor subfamily 1, group H, member 4 NR1I2 nuclear receptor subfamily 1, group I, member 2 NR1I3 nuclear receptor subfamily 1, group I, member 3 NR2C1 nuclear receptor subfamily 2, group C, member 1 NR2C2 nuclear receptor subfamily 2, group C, member 2 NR2E1 nuclear receptor subfamily 2, group E, member 1 NR2E3 nuclear receptor subfamily 2, group E, member 3 NR2F1 nuclear receptor subfamily 2, group F, member 1 NR2F2 nuclear receptor subfamily 2, group F, member 2 NR2F6 nuclear receptor subfamily 2, group F, member 6 NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) NR3C2 nuclear receptor subfamily 3, group C, member 2 NR4A1 nuclear receptor subfamily 4, group A, member 1 NR4A2 nuclear receptor subfamily 4, group A, member 2 NR4A3 nuclear receptor subfamily 4, group A, member 3 NR5A1 nuclear receptor subfamily 5, group A, member 1 NR5A2 nuclear receptor subfamily 5, group A, member 2 NR6A1 nuclear receptor subfamily 6, group A, member 1 NRIP1 nuclear receptor interacting protein 1 KAT2B K(lysine) acetyltransferase 2B PPARA peroxisome proliferator-activated receptor alpha PPARD peroxisome proliferator-activated receptor delta PPARG peroxisome proliferator-activated receptor gamma PPARGC1A peroxisome proliferator-activated receptor gamma, coactivator 1 alpha PPARGC1B peroxisome proliferator-activated receptor gamma, coactivator 1 beta PSMC3 proteasome (prosome, macropain) 26S subunit, ATPase, 3 PSMC5 proteasome (prosome, macropain) 26S subunit, ATPase, 5 RARA retinoic acid receptor, alpha RARB retinoic acid receptor, beta RARG retinoic acid receptor, gamma RBPJ recombination signal binding protein for immunoglobulin kappa J region RORA RAR-related orphan receptor A RORB RAR-related orphan receptor B RORC RAR-related orphan receptor C RXRA retinoid X receptor, alpha RXRB retinoid X receptor, beta RXRG retinoid X receptor, gamma THRA thyroid hormone receptor, alpha THRB thyroid hormone receptor, beta TRIP4 thyroid Hormone Receptor Interactor 4 ACTB Actin, beta B2M Beta-2-microglobulin GAPDH Glyceraldehyde-3-phosphate dehydrogenase GUSB Glucuronidase, beta HPRT1 Hypoxanthine phosphoribosyltransferase 1 Pgk1 Phosphoglycerate kinase 1 PPIA Peptidylprolyl isomerase A RPL13A Ribosomal protein L13a $595.00 |