Mouse Tnf qPCR primer set for tumor necrosis factor (TNF-alpha)

SYBR Green qPCR primers for detecting mouse Tnf (tumor necrosis factor, also called TNF-alpha) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Tnf qPCR Primer Set – tumor necrosis factor

Also known as: DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f

About this gene: According to NCBI Gene, mouse Tnf encodes a multifunctional proinflammatory cytokine in the tumor necrosis factor superfamily. The protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response and in regulating homeostasis, and it is also implicated in diseases of chronic inflammation. NCBI reports that deficiency of this gene in the mouse is associated with defects in the response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing produces multiple transcript variants. Mouse Tnf is a core qPCR target in mouse immunology, inflammation, infection, and cytokine-panel studies.

Gene details: NCBI Gene ID: 21926; UniProt: P06804; Chromosome location: 17 B1|17 18.59 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

Need Master Mix?