Mouse Tgfb1 qPCR primer set for transforming growth factor, beta 1 (TGF-beta1)

SYBR Green qPCR primers for detecting mouse Tgfb1 (transforming growth factor, beta 1, also called TGF-beta1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Tgfb1 qPCR Primer Set – transforming growth factor, beta 1

Also known as: TGF-beta1, TGFbeta1, Tgfb, Tgfb-1

About this gene: According to NCBI Gene, Tgfb1 encodes a secreted ligand of the TGF-beta superfamily. Ligands of this family bind various TGF-beta receptors, leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and it is found either in a latent form (a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein) or in an active form consisting of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. The protein regulates cell proliferation, differentiation, and growth, and can modulate expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects, and colon cancer. Mouse Tgfb1 is a frequent qPCR target in studies of fibrosis, wound healing, immune regulation, development, and tumor biology.

Gene details: NCBI Gene ID: 21803; UniProt: P04202; Chromosome location: 7 A3|7 13.98 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

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