Mouse Il6 qPCR primer set for interleukin 6 (IL-6)

SYBR Green qPCR primers for detecting mouse Il6 (interleukin 6, also called IL-6) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Il6 qPCR Primer Set – interleukin 6

Also known as: Il-6

About this gene: NCBI Gene states that mouse Il6 encodes a member of the interleukin family of cytokines with important functions in immune response, hematopoiesis, inflammation, and the acute phase response. Ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in the hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration, and wound healing. Alternative splicing produces multiple transcript variants encoding different isoforms. Mouse Il6 is a standard qPCR target in mouse models of inflammation, infection, autoimmunity, tissue injury, and cytokine profiling. It is often measured together with other inflammatory transcripts such as Tnf and Il1b.

Gene details: NCBI Gene ID: 16193; UniProt: P08505; Chromosome location: 5 B1|5 15.7 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

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