Mouse Il1b qPCR primer set for interleukin 1 beta (IL-1beta)

SYBR Green qPCR primers for detecting mouse Il1b (interleukin 1 beta, also called IL-1beta) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Il1b qPCR Primer Set – interleukin 1 beta

Also known as: IL-1beta, Il-1b

About this gene: NCBI Gene states that the Il1b protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. Mouse Il1b is commonly quantified by qPCR in studies of innate immunity, inflammasome activity, infection, autoimmunity, and inflammatory tissue injury, often alongside other cytokines such as Tnf and Il6. It is a standard readout of pro-inflammatory activation in cultured mouse cells, tissues, and in vivo disease models.

Gene details: NCBI Gene ID: 16176; UniProt: P10749; Chromosome location: 2 F1|2 62.97 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

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