Mouse Il10 qPCR primer set for interleukin 10 (IL-10)

SYBR Green qPCR primers for detecting mouse Il10 (interleukin 10, also called IL-10) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Il10 qPCR Primer Set – interleukin 10

Also known as: CSIF, If2a, Il-10

About this gene: NCBI Gene states that Il10 encodes an anti-inflammatory cytokine in the class-2 cytokine family. The protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It has also been shown to suppress autoimmune responses. The protein mediates its immunosuppressive signal through a specific interleukin 10 receptor complex. NCBI reports that aberrant functioning of this gene is associated with numerous immune disorders, including graft-versus-host disease and increased susceptibility to HIV-1 infection and rheumatoid arthritis. Mouse Il10 is commonly measured by qPCR when researchers are profiling anti-inflammatory or regulatory immune responses, often together with pro-inflammatory cytokines such as Tnf, Il6, and Ifng.

Gene details: NCBI Gene ID: 16153; UniProt: P18893; Chromosome location: 1 E4|1 56.89 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

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