Mouse Ifng qPCR primer set for interferon gamma (IFN-gamma)

SYBR Green qPCR primers for detecting mouse Ifng (interferon gamma, also called IFN-gamma) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Ifng qPCR Primer Set – interferon gamma

Also known as: IFN-g, If2f, Ifg

About this gene: According to NCBI Gene, Ifng encodes a soluble cytokine that is a member of the type II interferon class. The protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor, which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial, and parasitic infections and to several autoimmune diseases. Mouse Ifng is a core qPCR target in Th1 and cellular immune-response studies, infection models, and cytokine-profiling experiments, and it is often measured with other cytokines such as Tnf, Il6, and Il10.

Gene details: NCBI Gene ID: 15978; UniProt: P01580; Chromosome location: 10 D2|10 66.75 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets.

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