Mouse Gapdh qPCR primer set for glyceraldehyde-3-phosphate dehydrogenase

SYBR Green qPCR primers for detecting mouse Gapdh (glyceraldehyde-3-phosphate dehydrogenase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Gapdh qPCR Primer Set – glyceraldehyde-3-phosphate dehydrogenase

Also known as: Gapd

About this gene: According to NCBI Gene, mouse Gapdh encodes a member of the glyceraldehyde-3-phosphate dehydrogenase family and is a moonlighting protein with mechanistically distinct functions. It was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Later work has assigned additional functions, including nitrosylation of nuclear proteins, regulation of mRNA stability, and acting as a transferrin receptor on the macrophage cell surface. Alternative splicing produces multiple transcript variants, and many similar pseudogenes are found throughout the mouse genome. Mouse Gapdh is commonly used as a reference (housekeeping) gene to normalize qPCR data from mouse cells and tissues. It is also quantified in studies of glycolysis and of these additional Gapdh activities.

Gene details: NCBI Gene ID: 14433; UniProt: P16858; Chromosome location: 6 F2|6 59.32 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets, or profile Gapdh alongside related genes with our Housekeeping Gene Primer Sets.

Need Master Mix?