Mouse Actb qPCR primer set for actin, beta (beta-actin)

SYBR Green qPCR primers for detecting mouse Actb (actin, beta, also called beta-actin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Mouse Actb qPCR Primer Set – actin, beta

Also known as: Actx, E430023M04Rik, beta-actin

About this gene: NCBI Gene describes Actb as a member of the actin family. Actins are highly conserved, are among the most abundant proteins in eukaryotic cells, and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the Actb mRNA are regulated through the binding of multiple factors to its 3' UTR. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous Actb pseudogenes have been identified in the mouse genome. Mouse Actb is widely used as a reference (housekeeping) gene for normalizing qPCR data. It is also a target when researchers are studying the cytoskeleton or cell motility in mouse models.

Gene details: NCBI Gene ID: 11461; UniProt: P60710; Chromosome location: 5 G2|5 81.8 cM

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Mouse PCR Primer Sets, or profile Actb alongside related genes with our Housekeeping Gene Primer Sets.

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