Mouse PI3K-AKT Signaling Primer Library

Contains 88 primer sets directed against PI3K/AKT signaling genes and 8 housekeeping gene primer sets.

Provided in a 96-well microplate (20 ul - 10 uM)

Perform up to 100 PCR arrays (based on 20 ul assay volume per reaction)

Just add cDNA template and SYBR green master mix

GENE LIST:

Abl1 c-abl oncogene 1, receptor tyrosine kinase

Acaca acetyl-Coenzyme A carboxylase alpha

Acox1 acyl-Coenzyme A oxidase 1, palmitoyl

Aebp1 AE binding protein 1

Akt1 v-akt murine thymoma viral oncogene homolog 1

Akt2 v-akt murine thymoma viral oncogene homolog 2

Akt3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

Ang angiogenin, ribonuclease, RNase A family, 5

Araf v-raf murine sarcoma 3611 viral oncogene homolog

Bad BCL2-associated agonist of cell death

Bcl2 B-cell CLL/lymphoma 2

Bcl2L1 BCL2-like 1

Braf v-raf murine sarcoma viral oncogene homolog B1

Btk Bruton agammaglobulinemia tyrosine kinase

Cap1 CAP, adenylate cyclase-associated protein 1 (yeast)

Casp9 caspase 9, apoptosis-related cysteine peptidase

Cbl Cas-Br-M (murine) ecotropic retroviral transforming sequence

Ccnd1 cycline D1

Ccr2 chemokine (C-C motif) receptor 2

Ccr5 chemokine (C-C motif) receptor 5

Cd4 CD4 molecule

Cd40 CD40 molecule, TNF receptor superfamily member 5

Cdc42 cell division cycle 42 (GTP binding protein, 25kDa)

Cdh1 cadherin 1, type 1, E-cadherin (epithelial)

Cdkn1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Cdkn1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Cdkn2A cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

Creb1 cAMP responsive element binding protein 1

Ctnnb1 catenin (cadherin-associated protein), beta 1, 88kDa

Cxcr4 chemokine (C-X-C motif) receptor 4

E2F1 E2F transcription factor 1

Egfr epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Frap1 FKBP12-RAPAMYCIN COMPLEX-ASSOCIATED PROTEIN 1

Grb2 growth factor receptor-bound protein 2

Gsk3A glycogen synthase kinase 3 alpha

Gsk3B glycogen synthase kinase 3 beta

Gys1 glycogen synthase 1 (muscle)

Hk2 hexokinase 2

Hras v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Igf1 insulin-like growth factor 1 (somatomedin C)

Igf1R insulin-like growth factor 1 receptor

Igf2 insulin-like growth factor 2 (somatomedin A)

Igf2R insulin-like growth factor 2 receptor

Ins insulin

Insr insulin receptor

Irs1 insulin receptor substrate 1

Irs2 insulin receptor substrate 2

Irs4 insulin receptor substrate 4

Itgb1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

Itgb3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

Jak2 Janus kinase 2

Kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Lck lymphocyte-specific protein tyrosine kinase

Lep leptin

Map2K1 mitogen-activated protein kinase kinase 1

Mapk1 mitogen-activated protein kinase 1

Mapk14 mitogen-activated protein kinase 14

Mapk8 mitogen-activated protein kinase 8

Myc v-myc myelocytomatosis viral oncogene homolog (avian)

Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Nfkbia nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

Nos3 nitric oxide synthase 3 (endothelial cell)

Pak1 p21 protein (Cdc42/Rac)-activated kinase 1

Pde3B phosphodiesterase 3B, cGMP-inhibited

Pdpk1 3-phosphoinositide dependent protein kinase-1

Pik3C2B phosphoinositide-3-kinase, class 2, beta polypeptide

Pik3C3 phosphoinositide-3-kinase, class 3

Pik3Ca phosphoinositide-3-kinase, catalytic, alpha polypeptide

Pik3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

Pik3R4 phosphoinositide-3-kinase, regulatory subunit 4

Prkca protein kinase C, alpha

Prkce protein kinase C, epsilon

Prkcg protein kinase C, gamma

Pten phosphatase and tensin homolog

Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

Raf1 v-raf-1 murine leukemia viral oncogene homolog 1

Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian)

Rhoa ras homolog gene family, member A

Sgk1 serum/glucocorticoid regulated kinase 1

Shc1 SHC (Src homology 2 domain containing) transforming protein 1

Sos1 son of sevenless homolog 1 (Drosophila)

Spp1 secreted phosphoprotein 1

Src v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Syk spleen tyrosine kinase

Tlr4 toll-like receptor 4

Tnf tumor necrosis factor (TNF superfamily, member 2)

Actb actin, beta

B2M beta-2-microglobulin

Gapdh glyceraldehyde-phosphate-dehydrogenase

Gusb glucuronidase, beta

Hprt1 hypoxanthine phosphoribosyltransferase 1

Pgk1 phosphoglycerate kinase 1

Ppia peptidylprolyl isomerase A (cyclophilin A)

Rpl13A ribosomal protein L13a