Human TP53 qPCR primer set for tumor protein p53 (p53)

SYBR Green qPCR primers for detecting human TP53 (tumor protein p53, also called p53) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human TP53 qPCR Primer Set – tumor protein p53

Also known as: BCC7, BMFS5, LFS1, P53, TRP53

About this gene: According to NCBI Gene, TP53 encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing and the use of alternate promoters result in multiple transcript variants and isoforms, and additional isoforms can arise from alternate translation initiation codons. Human TP53 is a central qPCR target in cancer biology, DNA-damage response, cell-cycle control, and apoptosis studies, and it is often profiled together with p53-pathway genes such as CDKN1A (p21).

Gene details: NCBI Gene ID: 7157; HGNC ID: HGNC:11998; UniProt: P04637; RefSeq (MANE Select): NM_000546.6; Chromosome location: 17p13.1

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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