Human TNF qPCR primer set for tumor necrosis factor (TNF-alpha)

SYBR Green qPCR primers for detecting human TNF (tumor necrosis factor, also called TNF-alpha) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human TNF qPCR Primer Set – tumor necrosis factor

Also known as: DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F

About this gene: According to NCBI Gene, TNF encodes a multifunctional proinflammatory cytokine in the tumor necrosis factor superfamily. It is mainly secreted by macrophages and functions through the receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. The cytokine is involved in a wide spectrum of biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. NCBI reports that TNF has been implicated in autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis, ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice have also suggested a neuroprotective function. Human TNF is a core qPCR target in inflammation, immunology, infection, and cytokine-panel experiments.

Gene details: NCBI Gene ID: 7124; HGNC ID: HGNC:11892; UniProt: P01375; RefSeq (MANE Select): NM_000594.4; Chromosome location: 6p21.33

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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