Human CXCL8 qPCR primer set for C-X-C motif chemokine ligand 8 (IL-8)

SYBR Green qPCR primers for detecting human CXCL8 (C-X-C motif chemokine ligand 8, also called IL-8) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human CXCL8 qPCR Primer Set – C-X-C motif chemokine ligand 8

Also known as: GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8

About this gene: NCBI Gene describes the encoded protein as a member of the CXC chemokine family and a major mediator of the inflammatory response. IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. NCBI also notes roles in the proinflammatory signaling cascade and in systemic inflammatory response syndrome (SIRS), and discusses links to bronchiolitis, cystic fibrosis lung inflammation, coronary artery disease, and endothelial dysfunction. The protein can be secreted by tumor cells and is a potent angiogenic factor that can promote tumor migration, invasion, angiogenesis, and metastasis. Binding of IL-8 to IL-8RB/CXCR2 increases the permeability of blood vessels. Human IL8/CXCL8 is a common qPCR target in inflammation, infection, neutrophil biology, and tumor-microenvironment studies.

Gene details: NCBI Gene ID: 3576; HGNC ID: HGNC:6025; UniProt: P10145; RefSeq (MANE Select): NM_000584.4; Chromosome location: 4q13.3

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

Need Master Mix?