Human IL6 qPCR primer set for interleukin 6 (IL-6)

SYBR Green qPCR primers for detecting human IL6 (interleukin 6, also called IL-6) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human IL6 qPCR Primer Set – interleukin 6

Also known as: BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6

About this gene: NCBI Gene states that IL6 encodes a cytokine that functions in inflammation and the maturation of B cells. The protein has also been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. It is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. NCBI notes that IL6 function is implicated in a wide variety of inflammation-associated disease states, including susceptibility to diabetes mellitus and systemic juvenile rheumatoid arthritis, and that elevated levels of the encoded protein have been found in virus infections, including COVID-19. Human IL6 is a standard qPCR target in immunology, inflammation, infection, autoimmunity, and cytokine-profiling studies, and it is often measured together with other inflammatory transcripts.

Gene details: NCBI Gene ID: 3569; HGNC ID: HGNC:6018; UniProt: P05231; RefSeq (MANE Select): NM_000600.5; Chromosome location: 7p15.3

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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