Human IL1B qPCR primer set for interleukin 1 beta (IL-1beta)

SYBR Green qPCR primers for detecting human IL1B (interleukin 1 beta, also called IL-1beta) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human IL1B qPCR Primer Set – interleukin 1 beta

Also known as: IL-1, IL1-BETA, IL1F2, IL1beta

About this gene: According to NCBI Gene, IL1B encodes a member of the interleukin 1 cytokine family. The cytokine is produced by activated macrophages as a proprotein and is proteolytically processed to its active form by caspase 1 (CASP1/ICE). It is an important mediator of the inflammatory response and is involved in cell proliferation, differentiation, and apoptosis. NCBI notes that induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system contributes to inflammatory pain hypersensitivity, and that IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe COVID-19 have presented elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid, and lung damage induced by SARS-CoV-2 is to a large extent a result of the inflammatory response promoted by cytokines such as IL-1B. Human IL1B is a standard qPCR target in inflammasome, innate immunity, infection, autoimmunity, and cytokine-profiling studies.

Gene details: NCBI Gene ID: 3553; HGNC ID: HGNC:5992; UniProt: P01584; RefSeq (MANE Select): NM_000576.3; Chromosome location: 2q14.1

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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