Human IL10 qPCR primer set for interleukin 10 (IL-10)

SYBR Green qPCR primers for detecting human IL10 (interleukin 10, also called IL-10) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.

Human IL10 qPCR Primer Set – interleukin 10

Also known as: CSIF, GVHDS, IL-10, IL10A, TGIF

About this gene: NCBI Gene states that IL10 encodes a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. The cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages, and it also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-kappa B activity and is involved in regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested an essential immunoregulatory function in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Human IL10 is a common qPCR target in studies of anti-inflammatory and regulatory immune responses, autoimmunity, infection, and cytokine panels.

Gene details: NCBI Gene ID: 3586; HGNC ID: HGNC:5962; UniProt: P22301; RefSeq (MANE Select): NM_000572.3; Chromosome location: 1q32.1

This primer set has been experimentally validated and is SYBR Green compatible. The amplicon is 100-300 bp and produces a single melt peak.

What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H₂O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.

Amplicon size: Amplicons are 100-300 bp.

Representative amplification plot and melt curve (single peak), SYBR Green.

Browse all Human PCR Primer Sets.

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